ATP1A1 Rabbit Polyclonal Antibody

CAT#: TA383780

Phospho-alpha 1 Sodium Potassium ATPase (Ser23) Rabbit polyclonal Antibody

Product Images

Western Blot analysis of rat cells using Phospho-Na+/K+-ATPase α1 (S23) Polyclonal Antibody

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  Immunofluorescence analysis of NIH/3T3 cells, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.

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  Western blot analysis of lysates from rat brain, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: WB: 1/500-1/2000
  IF: 1/100-1/300
  ELISA: 1/5000
• Reactivities: Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from rat ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Ser23. AA range:15-64 (Phosphorylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):113
• Database Link:
  UniProt ID P06685
• Background: The ATPase Na+/K+ transporting subunit alpha 1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.