ATP5F1B Rabbit Monoclonal Antibody [Clone ID: R01-4G1]

CAT#: TA383785

ATPB Rabbit monoclonal Antibody

Product Images

Western blot detection of ATPB in Jurkat,Hela,COS7,Raji cell lysates using ATPB Rabbit mAb(1:1000 diluted).Predicted band size:57KDa.Observed band size:52KDa.

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  Immunohistochemistry of ATPB in paraffin-embedded Human tonsil using ATPB Rabbit mAb at dilution 1/20

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  Immunocytochemistry of ATPB(green) in Hela cells using ATPB Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R01-4G1
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/20-1/100
  ICC/IF: 1/50
  IP: 1/20-1/50
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human ATPB
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 57 kDa; Observed MW: 52 kDa
• Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• Database Link:
  Entrez Gene 506 Human
  UniProt ID P06576
• Background: Swiss-Prot Acc.P06576.Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
• Synonyms: ATPMB; ATPSB; MGC5231