Bcl2 Binding component 3 (BBC3) Rabbit Monoclonal Antibody [Clone ID: R05-9C7]

CAT#: TA383807

PUMA Rabbit monoclonal Antibody

Product Images

Western blot analysis of PUMA in K562 lysates using PUMA antibody.

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  Western blot analysis of PUMA in Jurkat, THP-1, C6, 3T3, Hela lysates using PUMA antibody

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  Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PUMA antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunocytochemistry analysis of PUMA (green) in 293T using PUMA antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R05-9C7
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20-1/100
  ICC/IF: 1/50-1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human PUMA
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 21 kDa; Observed MW: 21 kDa
• Gene Name: BCL2 binding component 3
• Database Link:
  Entrez Gene 27113 Human
  UniProt ID Q9BXH1
• Background: Swiss-Prot Acc.Q9BXH1.Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391). Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis (PubMed:23340338).
• Synonyms: FLJ42994; JFY-1; JFY1; PUMA