CASP4 Rabbit Polyclonal Antibody

CAT#: TA383878

Cleaved-Caspase 4/5 p20 Rabbit polyclonal Antibody

Product Images

Western blot analysis of Caspase 4/5 (p20, Cleaved-Asp270/Asp311) Antibody. The lane on the right is blocked with the Caspase 4/5 (p20, Cleaved-Asp270/Asp311) peptide.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 1/500-1/2000
  ELISA: 1/40000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 4/5. AA range:221-270 (Cleaved)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):47,22
• Database Link:
  UniProt ID P49662
• Background: Swiss-Prot Acc.P49662/P51878.CASP4 (caspase 4) encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, caspase 4 induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.