HP1 gamma (CBX3) Mouse Monoclonal Antibody [Clone ID: 9E5-1E3-3A8]

CAT#: TA383890

HP1 gamma (9E5) Mouse monoclonal Antibody

Product Images

Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1γ Mouse mAb diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1γ Mouse mAb diluted at 1:200.

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  Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1γ Mouse mAb diluted at 1:200.

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  Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1γ Mouse mAb diluted at 1:200.

Specifications

Product Data

• Clone Name: 9E5-1E3-3A8
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000-2000
  IHC: 1/100-200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant protein expressed in E.coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 24kDa
• Gene Name: chromobox 3
• Database Link:
  Entrez Gene 11335 Human
  UniProt ID Q13185
• Background: Swiss-Prot Acc.Q13185.Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitement of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).
• Synonyms: HECH; HP1-GAMMA; HP1Hs-gamma