HP1 alpha (CBX5) Mouse Monoclonal Antibody [Clone ID: 1E8-5C3-10E1]

CAT#: TA383895

HP1 alpha (1E8) Mouse monoclonal Antibody

Product Images

Western blot detection of HP1α in COS7,Hela cell lysates using HP1α (1E8) Mouse mAb(1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.

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  Western blot detection of HP1α in Hela,A549,HL-60,U2OS,C6 cell lysates using HP1α (1E8) antibody. Observed band size:22kDa.

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  Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α Mouse mAb diluted at 1:200.

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  Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α Mouse mAb diluted at 1:200.

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  Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 alpha; Mouse mAb diluted at 1:200

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Specifications

Product Data

• Clone Name: 1E8-5C3-10E1
• Applications: IHC, WB
• Recommended Dilution: WB: 1/500-1000
  IHC: 1/100-200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant protein expressed in E.coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 22kDa
• Gene Name: chromobox 5
• Database Link:
  Entrez Gene 23468 Human
  UniProt ID P45973
• Background: Swiss-Prot Acc.P45973.Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
• Synonyms: HP1; HP1-ALPHA; HP1A; HP1Hs-alpha