CD8A Rabbit Monoclonal Antibody [Clone ID: R09-8E7]

CAT#: TA383949

CD8 alpha Rabbit monoclonal Antibody

Product Images

Western blot detection of CD8 in THP-1 cell lysates using CD8 Rabbit mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:29KDa.

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  Western blot detection of CD8 in K562,CHO-K1,Hela cell lysates using CD8 Rabbit mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:29KDa.

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  Immunohistochemistry of CD8 in paraffin-embedded Human tonsil using CD8 Rabbit mAb at dilution 1/1

Specifications

Product Data

• Clone Name: R09-8E7
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50-1/200
• Reactivities: Hamster, Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human CD8
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 26 kDa; Observed MW: 29 kDa
• Gene Name: CD8a molecule
• Database Link:
  Entrez Gene 925 Human
  UniProt ID P01732
• Background: Swiss-Prot Acc.P01732.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
• Synonyms: CD8; Leu2; MAL; OTTHUMP00000203721; p32