CDKN1A Rabbit Monoclonal Antibody [Clone ID: R06-3A5]

CAT#: TA383976

p150 CAF1 Rabbit monoclonal Antibody

Product Images

Western blot detection of p150 CAF1 in K562 cell lysates using p150 CAF1 Rabbit mAb(1:1000 diluted).Predicted band size:107kDa.Observed band size:150kDa.

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  Immunocytochemistry of p150 CAF1(green) in Hela cells using p150 CAF1 Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of p15 CAF1/CAF in paraffin-embedded Human lung cancer tissue using p15 CAF1/CAF Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R06-3A5
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
  ICC/IF: 1/200-1/1000
  IP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human p150 CAF1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 107 kDa; Observed MW: 150 kDa
• Database Link:
  UniProt ID Q13111
• Background: Swiss-Prot Acc.Q13111.Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci .