p21 (CDKN1A) Rabbit Monoclonal Antibody [Clone ID: R07-3K5]

CAT#: TA383977

p21 Rabbit monoclonal Antibody

Product Images

Western blot detection of p21 in MCF-7 cell lysates using p21 Rabbit mAb(1:1000 diluted).Predicted band size:18KDa.Observed band size:21KDa.

Specifications

Product Data

• Clone Name: R07-3K5
• Applications: WB
• Recommended Dilution: WB: 1/1000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human p21
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 18 kDa; Observed MW: 21 kDa
• Gene Name: cyclin-dependent kinase inhibitor 1A
• Database Link:
  Entrez Gene 1026 Human
  UniProt ID P38936
• Background: Swiss-Prot Acc.P38936.May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
• Synonyms: CAP20; CDKN1; CIP1; MDA-6; MDA6; OTTHUMP00000016298; P21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1; WAF1