Cortactin (CTTN) Rabbit Monoclonal Antibody [Clone ID: R07-2C5]

CAT#: TA384062

Cortactin Rabbit monoclonal Antibody

Product Images

Western blot detection of Cortactin in K562,Rat Brain,C6,3T3,Hela cell lysates using Cortactin Rabbit mAb(1:1000 diluted).Predicted band size:62kDa.Observed band size:80-85kDa.

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  Immunohistochemistry of Cortactin in paraffin-embedded Human breast cancer tissue using Cortactin Rabbit mAb at dilution 1/20

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  Immunocytochemistry of Cortactin(green) in Hela cells using Cortactin Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R07-2C5
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20-1/100
  ICC/IF: 1/200
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Cortactin
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 62 kDa; Observed MW: 80-85 kDa
• Gene Name: cortactin
• Database Link:
  Entrez Gene 2017 Human
  UniProt ID Q14247
• Background: Swiss-Prot Acc.Q14247.Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones . Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density . Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290). Plays a role in focal adhesion assembly and turnover . In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits . Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454).
• Synonyms: 1110020L01Rik; Amplaxin; cortactin; EMS1; FLJ34459