DDIT3 Rabbit Monoclonal Antibody [Clone ID: R02-6A3]

CAT#: TA384090

DDIT3 Rabbit monoclonal Antibody

Product Images

Western blot detection of DDIT3 in 3T3 cell lysates using DDIT3 Rabbit mAb(1:1000 diluted).Predicted band size:19KDa.Observed band size:27KDa.

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  Western blot detection of DDIT3 in K562,CHO-K1,Hela cell lysates using DDIT3 Rabbit mAb(1:1000 diluted).Predicted band size:19KDa.Observed band size:27KDa.

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  Immunohistochemistry of DDIT3 in paraffin-embedded Human breast cancer tissue using DDIT3 Rabbit mAb at dilution 1/1

Specifications

Product Data

• Clone Name: R02-6A3
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000-1/2000
  IHC: 1/20-1/50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human DDIT3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 19 kDa; Observed MW: 27 kDa
• Gene Name: DNA damage inducible transcript 3
• Database Link:
  Entrez Gene 1649 Human
  UniProt ID P35638
• Background: Swiss-Prot Acc.P35638.Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.
• Synonyms: CEBPZ; CHOP; CHOP10; DDIT-3; GADD153; MGC4154