Desmin (DES) Rabbit Polyclonal Antibody

CAT#: TA384095

Desmin Rabbit polyclonal Antibody

Product Images

Western Blot analysis of various cells using Desmin Polyclonal Antibody diluted at 1：2000

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  Western Blot analysis of VEC cells using Desmin Polyclonal Antibody diluted at 1：2000

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  The picture was kindly provided by our customer

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  Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Desmin Antibody. The picture on the right is blocked with the synthesized peptide.

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  Western blot analysis of lysates from K562 cells, using Desmin Antibody. The lane on the right is blocked with the synthesized peptide.

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  IF: 1/200-1/1000
  ELISA: 1/10000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human Desmin. AA range:421-470
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):55
• Gene Name: desmin
• Database Link:
  Entrez Gene 1674 Human
  UniProt ID P17661
• Background: Swiss-Prot Acc.P17661.Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin .
• Synonyms: CMD1I; CSM1; CSM2; desmin; FLJ12025; FLJ39719; FLJ41013; FLJ41793