EGFR Rabbit Monoclonal Antibody [Clone ID: R05-2A2]

CAT#: TA384139

Phospho-EGFR (Tyr1092) Rabbit monoclonal Antibody

Product Images

Western blot detection of EGFR (Phospho-Tyr1092) in C6,3T3,Hela cell lysates using EGFR (Phospho-Tyr1092) Rabbit mAb(1:1000 diluted).Predicted band size:134kDa.Observed band size:175kDa.

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  Immunohistochemistry of Phospho-EGFR (Tyr1092) in paraffin-embedded Human breast cancer tissue using Phospho-EGFR (Tyr1092) Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R05-2A2
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Tyr1092 of human EGFR (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 134 kDa; Observed MW: 175 kDa
• Gene Name: epidermal growth factor receptor
• Database Link:
  Entrez Gene 1956 Human
  UniProt ID P00533
• Background: Swiss-Prot Acc.P00533.Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance .
• Synonyms: ERBB; ERBB1; HER1; mENA; PIG61