PHD3 (EGLN3) Rabbit Monoclonal Antibody [Clone ID: R04-3K9]

CAT#: TA384143

PHD3 Rabbit monoclonal Antibody

Product Images

Western blot detection of PHD3 in Rat lung and Mouse lung lysates using PHD3 Rabbit mAb (1:1000 diluted).Predicted band size:27kDa.Observed band size:27kDa.

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  Immunohistochemistry of PHD3 in paraffin-embedded Human colon cancer tissue using PHD3 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R04-3K9
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human PHD3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 27 kDa; Observed MW: 27 kDa
• Gene Name: egl-9 family hypoxia inducible factor 3
• Database Link:
  Entrez Gene 112399 Human
  UniProt ID Q9H6Z9
• Background: Swiss-Prot Acc.Q9H6Z9.Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.
• Synonyms: FLJ21620; HIF-PH3; HIFPH3; HPH-1; MGC125998; MGC125999; PHD3