EIF3B Rabbit Monoclonal Antibody [Clone ID: R09-4I5]

CAT#: TA384153

eIF3B Rabbit monoclonal Antibody

Product Images

Western blot detection of eIF3B in Raji,COS7,3T3,Hela cell lysates using eIF3B Rabbit mAb(1:1000 diluted).Predicted band size:93KDa.Observed band size:117KDa.

• 

  Immunocytochemistry of eIF3B(green) in Hela cells using eIF3B Rabbit mAb at dilution 1/50, and DAPI(blue)

Specifications

Product Data

• Clone Name: R09-4I5
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  ICC/IF: 1/20-1/50
  IP: 1/20-1/50
• Reactivities: Human, Monkey, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human eIF3B
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 93 kDa; Observed MW: 117 kDa
• Gene Name: eukaryotic translation initiation factor 3 subunit B
• Database Link:
  Entrez Gene 8662 Human
  UniProt ID P55884
• Background: Swiss-Prot Acc.P55884.RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
• Synonyms: eIF-3-eta; EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; hPrt1; MGC104664; MGC131875; PRT1