EIF4A1 Mouse Monoclonal Antibody [Clone ID: 6D7-10D5-7H7]

CAT#: TA384154

eIF4A1 (6D7) Mouse monoclonal Antibody

Product Images

Western blot detection of eIF4A1 in T47D,3T3,COS7,C6 and Hela cell lysates using eIF4A1 mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.

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  IF analysis of Hela with (Left) and DAPI (Right) diluted at 1:100

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  Western blot detection of eIF4A (6D7) in CHO-K1,Rat brain,C6,3T3,Hela cell lysates using eIF4A (6D7) antibody. Observed band size:48kDa.

Specifications

Product Data

• Clone Name: 6D7-10D5-7H7
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000-3000
  ICC: 1/100-200
  IHC: 1/50-300
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic Peptide of eIF4A1
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):48
• Gene Name: eukaryotic translation initiation factor 4A1
• Database Link:
  Entrez Gene 1973 Human
  UniProt ID P60842
• Background: Swiss-Prot Acc.P60842.ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
• Synonyms: DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I