Her2 (ERBB2) Rabbit Polyclonal Antibody

CAT#: TA384184

Phospho-ErbB 2 (Tyr1221/Tyr1222) Rabbit polyclonal Antibody

Product Images

Western Blot analysis of various cells using Phospho-Neu (Y1221/Y122,2) Polyclonal Antibody

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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr122,2) Antibody. The picture on the right is blocked with the phospho peptide.

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  Western blot analysis of lysates from SK-OV3 cells treated with EGF, using HER2 (Phospho-Tyr1221/Tyr122,2) Antibody. The lane on the right is blocked with the phospho peptide.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  Immunoprecipitation: 2-5
  ELISA: 1/20000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222. AA range:1191-1240 (Phosphorylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):180
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  Entrez Gene 2064 Human
  UniProt ID P04626
• Background: Swiss-Prot Acc.P04626.Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
• Synonyms: C-erbB-2; CD340; erbB-2; HER-2; HER-2/neu; HER2; herstatin; NEU; NGL; p185erbB2; TKR1