IRE1 (ERN2) Rabbit Monoclonal Antibody [Clone ID: R03-3I5]

CAT#: TA384188

Phospho-IRE1 (Ser724) Rabbit monoclonal Antibody

Product Images

Western blot detection of IRE1 (Phospho-Ser724) in K562 cell lysates using IRE1 (Phospho-Ser724) Rabbit mAb(1:1000 diluted).Predicted band size:110kDa.Observed band size:110kDa.

Specifications

Product Data

• Clone Name: R03-3I5
• Applications: WB
• Recommended Dilution: WB: 1/1000-1/5000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser724 of human IRE1 (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 110 kDa; Observed MW: 110 kDa
• Gene Name: endoplasmic reticulum to nucleus signaling 2
• Database Link:
  Entrez Gene 10595 Human
  UniProt ID O75460
• Background: Swiss-Prot Acc.O75460.Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262).
• Synonyms: hIRE2p; Ire1-beta; IRE1b; IRE2