Estrogen Receptor 1 (ESR1) Rabbit Monoclonal Antibody [Clone ID: R05-1E4]

CAT#: TA384190

Estrogen Receptor alpha Rabbit monoclonal Antibody

Product Images

Western blot detection of Estrogen Receptor alpha in K562,Hela cell lysates using Estrogen Receptor alpha Rabbit mAb(1:1000 diluted).Predicted band size:66kDa.Observed band size:66kDa.

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  Immunohistochemistry of Estrogen Receptor alpha in paraffin-embedded Human breast cancer tissue using Estrogen Receptor alpha Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R05-1E4
• Applications: ChIP, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
  ICC/IF: 1/500
  IP: 1/20
  ChIP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Estrogen Receptor alpha
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 66 kDa; Observed MW: 66 kDa
• Gene Name: estrogen receptor 1
• Database Link:
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: Swiss-Prot Acc.P03372.Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
• Synonyms: DKFZp686N23123; ER; ER-alpha; Era; ESR; ESRA; NR3A1