Fibrillarin (FBL) Rabbit Monoclonal Antibody [Clone ID: R09-7E5]

CAT#: TA384212

Fibrillarin Rabbit monoclonal Antibody

Product Images

Western blot detection of Fibrillarin in K562,C6,3T3 cell lysates using Fibrillarin Rabbit mAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:34kDa.

Specifications

Product Data

• Clone Name: R09-7E5
• Applications: IP, WB
• Recommended Dilution: WB: 1/1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Fibrillarin
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 34 kDa; Observed MW: 34 kDa
• Gene Name: fibrillarin
• Database Link:
  Entrez Gene 2091 Human
  UniProt ID P22087
• Background: Swiss-Prot Acc.P22087.S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
• Synonyms: FIB; FIB1; fibrillarin; FLRN; RNU3IP1