FOXO1 Rabbit Monoclonal Antibody [Clone ID: R07-7C4]

CAT#: TA384252

FOXO1 Rabbit monoclonal Antibody

Product Images

Western blot detection of FOXO1A in HepG2 cell lysates using FOXO1A Rabbit mAb(1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

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  Immunohistochemistry of FOXO1A in paraffin-embedded Human tonsil using FOXO1A Rabbit mAb at dilution 1/100

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  Immunocytochemistry of FOXO1A(green) in Hela cells using FOXO1A Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R07-7C4
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000-1/5000
  IHC: 1/50-1/200
  ICC/IF: 1/20-1/50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human FOXO1A
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 70 kDa; Observed MW: 70 kDa
• Gene Name: forkhead box O1
• Database Link:
  Entrez Gene 2308 Human
  UniProt ID Q12778
• Background: Swiss-Prot Acc.Q12778.Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling .
• Synonyms: FKH1; FKHR; FOXO1A