Histone H2A.X (H2AFX) Rabbit Monoclonal Antibody [Clone ID: R02-5E6]

CAT#: TA384365

Histone H2A.X Rabbit monoclonal Antibody

Product Images

Western blot detection of Histone H2A.X in 3T3,Hela cell lysates using Histone H2A.X Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:15kDa.

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  Immunohistochemistry of Histone H2A.X in paraffin-embedded Human colon cancer tissue using Histone H2A.X Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R02-5E6
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000-1/5000
  IHC: 1/200
  ICC/IF: 1/200
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Histone H2A.X
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Gene Name: H2A histone family member X
• Database Link:
  Entrez Gene 3014 Human
  UniProt ID P16104
• Background: Swiss-Prot Acc.P16104.Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
• Synonyms: H2A.X; H2A/X; H2AX