H2BC21 Rabbit Polyclonal Antibody

CAT#: TA384373

Acetyl-Histone H2B (Lys15) Rabbit polyclonal Antibody

Product Images

Western blot - Acetyl-Histone H2B-K15 pAb Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Exposure time: 10s.

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  Immunohistochemistry - Acetyl-Histone H2B-K15 pAb Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody at dilution of 1:100 (40x lens).

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  Immunohistochemistry - Acetyl-Histone H2B-K15 pAb Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody at dilution of 1:1 (4x lens).

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  Immunohistochemistry - Acetyl-Histone H2B-K15 pAb Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody at dilution of 1:100 (40x lens).

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  Immunohistochemistry - Acetyl-Histone H2B-K15 pAb Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody at dilution of 1:100 (40x lens).

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  Immunofluorescence - Acetyl-Histone H2B-K15 pAb Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  IP: 1/50-1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: AA synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B (Acetylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa): 15
• Gene Name: histone cluster 2, H2be
• Database Link:
  Entrez Gene 8349 Human
  UniProt ID P33778
• Synonyms: GL105; H2B; H2B-GL105; H2B.1; H2B.q; H2B/q; H2BFQ; H2BGL105; H2BQ; MGC119802; MGC119804; MGC129733; MGC129734