H3FT (HIST3H3) Rabbit Polyclonal Antibody

CAT#: TA384380

Phospho-Histone H3 (Ser10) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from HeLa cells,untreated or 1mM hydroxyurea treated for 5 hours,using Phospho-Histone H3(Ser10) Rabbit pAb(1:1000 diluted).

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  Immunofluorescent analysis of Hela cells labeled with Phospho-Histone H3(Ser10) (dilution 1:200) Rabbit pAb. DAPI was used to stain nucleus(blue).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1/1000
  ICC: 1/200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human Phospho-Histone H3(Ser10) synthetic peptide conjugated to KLH. (Phosphorylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 17kDa
• Gene Name: histone cluster 3, H3
• Database Link:
  Entrez Gene 8290 Human
  UniProt ID P68431
• Background: Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Synonyms: H3.4; H3/g; H3/t; H3FT; H3t; MGC126886; MGC126888