H3FT (HIST3H3) Rabbit Monoclonal Antibody [Clone ID: R03-7K5]

CAT#: TA384387

Phospho-Histone H3 (Ser28) Rabbit monoclonal Antibody

Product Images

Western blot detection of Histone H3 (Phospho-Ser28) in 3T3,Hela cell lysates using Histone H3 (Phospho-Ser28) Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:15kDa.

• 

  Immunohistochemistry of Histone H3 (Phospho-Ser28) in paraffin-embedded Human colon cancer tissue using Histone H3 (Phospho-Ser28) Rabbit mAb at dilution 1/50

• 

  Immunocytochemistry of Histone H3 (Phospho-Ser28)(green) in Hela cells using Histone H3 (Phospho-Ser28) Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R03-7K5
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
  ICC/IF: 1/50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser28 of human Histone H3 (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Gene Name: histone cluster 3, H3
• Database Link:
  Entrez Gene 8290 Human
  UniProt ID P68431
• Background: Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).CautionThe original paper reporting lysine deamination at Lys-5 by LOXL2 has been retracted due to inappropriate manipulation of figure data (PubMed:22483618, PubMed:27392148). However, this modification was confirmed in a subsequent publication (PubMed:27735137).
• Synonyms: H3.4; H3/g; H3/t; H3FT; H3t; MGC126886; MGC126888