H3FT (HIST3H3) Mouse Monoclonal Antibody [Clone ID: 9E3-5A9-8G2]

CAT#: TA384425

MonoMethyl-Histone H3 (Lys79) (9E3) Mouse monoclonal Antibody

Product Images

Western blot analysis of Hela, diluted at 1) 1:1000 . 2) 1:3000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

• 

  Western blot detection of Mono-Methyl-Histone H3 in 293,Hela cell lysates using Mono-Methyl-Histone H3 (Lys79) (9E3) Mouse mAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Specifications

Product Data

• Clone Name: 9E3-5A9-8G2
• Applications: WB
• Recommended Dilution: WB: 1/500-2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic Peptide of Histone H3 (Mono Methyl Lys79) (Methylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):15
• Gene Name: histone cluster 3, H3
• Database Link:
  Entrez Gene 8290 Human
  UniProt ID P68431
• Background: Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).
• Synonyms: H3.4; H3/g; H3/t; H3FT; H3t; MGC126886; MGC126888