H3FT (HIST3H3) Rabbit Polyclonal Antibody

CAT#: TA384429

MonoMethyl-Histone H3 (Lys4) Rabbit polyclonal Antibody

Product Images

Western blot - MonoMethyl-Histone H3-K4 pAb Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K4 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

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  - MonoMethyl-Histone H3-K4 pAb Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K4 antibody .

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  Immunohistochemistry - MonoMethyl-Histone H3-K4 pAb Immunohistochemistry of paraffin-embedded rat spleen using MonoMethyl-Histone H3-K4 antibody at dilution of 1:2 (4x lens).

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  Immunohistochemistry - MonoMethyl-Histone H3-K4 pAb Immunohistochemistry of paraffin-embedded human thyroid cancer using MonoMethyl-Histone H3-K4 antibody at dilution of 1:200 (40x lens).

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  Immunofluorescence - MonoMethyl-Histone H3-K4 pAb Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K4 antibody . Blue: DAPI for nuclear staining.

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  Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K4 pAb Chromatin immunoprecipitation analysis of extracts of 293T cells, using MonoMethyl-Histone H3-K4 antibody and rabbit IgG. P1 and P2 were located on promoter (GAPDH). The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Specifications

Product Data

• Applications: ChIP, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  IF: 1/50-1/200
  IP: 1/50-1/200
  ChIP: 1/20-1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3 (Methylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW:15kDa; Observed MW:18kDa
• Gene Name: histone cluster 3, H3
• Database Link:
  Entrez Gene 8290 Human
  UniProt ID Q16695
• Background: Swiss-Prot Acc.Q16695.
• Synonyms: H3.4; H3/g; H3/t; H3FT; H3t; MGC126886; MGC126888