H3FT (HIST3H3) Rabbit Polyclonal Antibody

CAT#: TA384430

TriMethyl-Histone H3 (Lys14) Rabbit polyclonal Antibody

Product Images

Western blot - TriMethyl-Histone H3-K14 pAb Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K14 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

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  Immunofluorescence - TriMethyl-Histone H3-K14 pAb Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K14 pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ChIP, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  IF: 1/50-1/200
  IP: 1/50-1/200
  ChIP: 1/20-1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human TriMethyl-Histone H3-K14 (Methylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW:15kDa; Observed MW:18kDa
• Gene Name: histone cluster 3, H3
• Database Link:
  Entrez Gene 8290 Human
  UniProt ID Q16695
• Background: Swiss-Prot Acc.Q16695.
• Synonyms: H3.4; H3/g; H3/t; H3FT; H3t; MGC126886; MGC126888