GRP78 (HSPA5) Mouse Monoclonal Antibody [Clone ID: 4F11-D5-C10]

CAT#: TA384460

GRP78 BiP (4F11) Mouse monoclonal Antibody

Product Images

Western blot analysis of extracts from 3T3,A549,C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.

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  Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20u2103 and using BiP/GRP78 mouse mAb (dilution 1:200).

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  Immunoprecipitation of BiP/GRP78 from Hela cell extracts using BiP/GRP78 Mouse mAb.Western blot was performed using BiP/GRP78 Mouse mAb.

Specifications

Product Data

• Clone Name: 4F11-D5-C10
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/1000
  ICC: 1/200
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant human BiP/GRP78 protein expressed in E.coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 78kDa
• Gene Name: heat shock protein family A (Hsp70) member 5
• Database Link:
  Entrez Gene 3309 Human
  UniProt ID P11021
• Background: Swiss-Prot Acc.P11021.Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
• Synonyms: BiP; FLJ26106; GRP78; MIF2