IFITM3 Rabbit Monoclonal Antibody [Clone ID: R09-6I5]

CAT#: TA384482

Fragilis Rabbit monoclonal Antibody

Product Images

Western blot detection of Fragilis in K562,Hela cell lysates using Fragilis Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:15kDa.

• 

  Immunocytochemistry of Fragilis(green) in Hela cells using Fragilis Rabbit mAb at dilution 1/50, and DAPI(blue)

• 

  Immunohistochemistry of Fragilis in paraffin-embedded Human breast cancer tissue using Fragilis Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R09-6I5
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
  ICC/IF: 1/50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Fragilis
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Gene Name: interferon induced transmembrane protein 3
• Database Link:
  Entrez Gene 10410 Human
  UniProt ID Q01628
• Background: Swiss-Prot Acc.Q01628.IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.
• Synonyms: 1-8U; interferon-inducible; IP15