IL1 beta (IL1B) Rabbit Polyclonal Antibody

CAT#: TA384520

IL-1 beta Rabbit polyclonal Antibody

Product Images

Western blot - IL1B Polyclonal Antibody. Western blot analysis of extracts of various cell lines, using IL1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 3s.

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  Western blot - IL1B Polyclonal Antibody. Western blot analysis of extracts of THP-1 cells, using IL1B antibody at 1:1000 dilution. THP-1 cells were treated by PMA at 37℃ for overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 3s.

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  Immunohistochemistry - IL1B Polyclonal Antibody. Immunohistochemistry of paraffin-embedded rat lung using IL1B antibody at dilution of 1:1 .

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  Immunofluorescence - IL1B Polyclonal Antibody. Immunofluorescence analysis of THP-1 cells using IL1B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence - IL1B Polyclonal Antibody. Immunofluorescence analysis of mouse lung using IL1B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  IF: 1/50-1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human IL1B.Email for sequence
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW:30kDa;Observed MW:17kDa, 31kDa
• Gene Name: interleukin 1 beta
• Database Link:
  Entrez Gene 3553 Human
  UniProt ID P01584
• Background: Swiss-Prot Acc.P01584.The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
• Synonyms: catabolin; IL-1; IL-1B; IL1-BETA; IL1F2; pro-interleukin-1-beta