ILF3 Rabbit Monoclonal Antibody [Clone ID: R04-3C1]

CAT#: TA384531

ILF3 Rabbit monoclonal Antibody

Product Images

Western blot detection of ILF3/NF90 in Jurkat,C6,Hela cell lysates using ILF3/NF90 Rabbit mAb(1:1000 diluted).Predicted band size:95kDa.Observed band size:95kDa.

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  Immunohistochemistry of ILF3 in paraffin-embedded Human lung cancer tissue using ILF3 Rabbit mAb at dilution 1/50

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  Immunocytochemistry of ILF3 (green) in hela using ILF3 Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R04-3C1
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/2000-1/20000
  IHC: 1/50
  ICC/IF: 1/100
  IP: 1/20
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human ILF3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 95 kDa; Observed MW: 95 kDa
• Gene Name: interleukin enhancer binding factor 3
• Database Link:
  Entrez Gene 3609 Human
  UniProt ID Q12906
• Background: Swiss-Prot Acc.Q12906.RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552).
• Synonyms: CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF90; NF110; NFAR; NFAR-1; NFAR2; TCP80; TCP110