c-Jun (JUN) Rabbit Monoclonal Antibody [Clone ID: R02-1G3]

CAT#: TA384583

c-Jun Rabbit monoclonal Antibody

Product Images

Western blot detection of c-Jun in 3T3 cell lysates using c-Jun Rabbit mAb(1:1000 diluted).Predicted band size:36KDa.Observed band size:48,43KDa.

Specifications

Product Data

• Clone Name: R02-1G3
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/50
  IP: 1/20
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human c-Jun
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 36 kDa; Observed MW: 48,43 kDa
• Gene Name: Jun proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  Entrez Gene 3725 Human
  UniProt ID P05412
• Background: Swiss-Prot Acc.P05412.Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
• Synonyms: AP-1; AP1; c-Jun; p39