KDM1A Rabbit Monoclonal Antibody [Clone ID: R08-3J5]

CAT#: TA384594

KDM1A Rabbit monoclonal Antibody

Product Images

Western blot detection of KDM1/LSD1 in Jurkat cell lysates using KDM1/LSD1 Rabbit mAb(1:1000 diluted).Predicted band size:93KDa.Observed band size:110KDa.

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  Western blot detection of KDM1/LSD1 in Hela,CHO-K1,C6,Jurkat cell lysates using KDM1/LSD1 Rabbit mAb(1:500 diluted).Predicted band size:93kDa.Observed band size:110kDa.

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  Immunocytochemistry of KDM1/LSD1(green) in Hela cells using KDM1/LSD1 Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R08-3J5
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  ICC/IF: 1/20-1/100
  IP: 1/20-1/50
• Reactivities: Hamster, Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human KDM1/LSD1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 93 kDa; Observed MW: 110 kDa
• Gene Name: lysine demethylase 1A
• Database Link:
  Entrez Gene 23028 Human
  UniProt ID O60341
• Background: Swiss-Prot Acc.O60341.Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
• Synonyms: AOF2; BHC110; KDM1; LSD1