KDM4B Rabbit Monoclonal Antibody [Clone ID: R08-9G9]

CAT#: TA384596

KDM4B Rabbit monoclonal Antibody

Product Images

Western blot detection of KDM4B in 293,COS7 cell lysates using KDM4B/JMJD2B Rabbit mAb(1:1000 diluted).Predicted band size:122KDa.Observed band size:150KDa.

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  Immunohistochemistry of KDM4B/JMJD2B in paraffin-embedded Human lung cancer tissue using KDM4B/JMJD2B Rabbit mAb at dilution 1/20

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  Immunocytochemistry of KDM4B/JMJD2B(green) in Hela cells using KDM4B/JMJD2B Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R08-9G9
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
  ICC/IF: 1/2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human KDM4B/JMJD2B
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 122 kDa; Observed MW: 150 kDa
• Gene Name: lysine demethylase 4B
• Database Link:
  Entrez Gene 23030 Human
  UniProt ID O94953
• Background: Swiss-Prot Acc.O94953.Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
• Synonyms: FLJ44906; JHDM3B; JMJD2B; KIAA0876