Cytokeratin 17 (KRT17) Mouse Monoclonal Antibody [Clone ID: 8C6-4H2-5C1]

CAT#: TA384625

Cytokeratin 17 (8C6) Mouse monoclonal Antibody

Product Images

Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, diluted at 1:2000.

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  1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200

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  Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CK17 Monoclonal Antibody(10A1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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  Immunofluorescence analysis of Human-breast tissue. 1,CK17 Monoclonal Antibody(10A1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Specifications

Product Data

• Clone Name: 8C6-4H2-5C1
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IP: 1/200
  IF: 1/200
  IHC: 1/50-300
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic Peptide of CK17
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):48
• Gene Name: keratin 17
• Database Link:
  Entrez Gene 3872 Human
  UniProt ID Q04695
• Background: Swiss-Prot Acc.Q04695.Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair . Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state . Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway . Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial
• Synonyms: 39.1; CK-17; Cytokeratin-17; K17; Keratin-17; PC; PC2; PCHC1