Cytokeratin 18 (KRT18) Rabbit Monoclonal Antibody [Clone ID: R03-5E3]

CAT#: TA384628

Cytokeratin 18 Rabbit monoclonal Antibody

Product Images

Western blot detection of Cytokeratin 18 in K562,Hela cell lysates using Cytokeratin 18 Rabbit mAb(1:1000 diluted).Predicted band size:48kDa.Observed band size:48kDa.

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  Immunohistochemistry of Cytokeratin 18 in paraffin-embedded Human colon cancer tissue using Cytokeratin 18 Rabbit mAb at dilution 1/50

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  Immunocytochemistry of Cytokeratin 18(green) in Hela cells using Cytokeratin 18 Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R03-5E3
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000
  IHC: 1/50
  ICC/IF: 1/50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Cytokeratin 18
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 48 kDa; Observed MW: 48 kDa
• Gene Name: keratin 18
• Database Link:
  Entrez Gene 3875 Human
  UniProt ID P05783
• Background: Swiss-Prot Acc.P05783.Involved in the uptake of thrombin-antithrombin complexes by hepatic cells . When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
• Synonyms: CK-18; CYK18; Cytokeratin-18; K18; Keratin-18