Cytokeratin 6C (KRT6C) Mouse Monoclonal Antibody [Clone ID: 1A5-9F5-6E9]

CAT#: TA384633

Cytokeratin 6 (1A5) Mouse monoclonal Antibody

Product Images

Western blot detection of Cytokeratin 6 (1A5) in Hela,C6，3T3，K562，HepG2 cell lysates using Cytokeratin 6 (1A5) antibody. Observed band size:56-6kDa.

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  Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,CytokeRatin 6 Monoclonal Antibody(4A1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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  Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cytokeratin 6 Monoclonal Antibody(4A1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Specifications

Product Data

• Clone Name: 1A5-9F5-6E9
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  IF: 1/200
  IHC: 1/50-300
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic Peptide of Cytokeratin 6
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):56-60
• Gene Name: keratin 6C
• Database Link:
  Entrez Gene 286887 Human
  UniProt ID P02538
• Background: Swiss-Prot Acc.P02538.Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.
• Synonyms: Cytokeratin-6C; Cytokeratin-6E; K6E; KRT6E; MGC102925; MGC163455; MGC163457