LAMP2 Rabbit Monoclonal Antibody [Clone ID: R03-2J8]

CAT#: TA384644

LAMP2A Rabbit monoclonal Antibody

Product Images

Western blot detection of LAMP2 in 3T3 cell lysates using LAMP2 Rabbit mAb(1:1000 diluted).Predicted band size:45kDa.Observed band size:110kDa.

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  Immunohistochemistry of LAMP2 in paraffin-embedded Human lung cancer tissue using LAMP2 Rabbit mAb at dilution 1/20

Specifications

Product Data

• Clone Name: R03-2J8
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20-1/1000
  IP: 1/20
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human LAMP2A
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 45 kDa; Observed MW: 110 kDa
• Gene Name: lysosomal associated membrane protein 2
• Database Link:
  Entrez Gene 3920 Human
  UniProt ID P13473
• Background: Swiss-Prot Acc.P13473.Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125). Plays a role in lysosomal protein degradation in response to starvation . Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820). Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820).
• Synonyms: CD107b; LAMP-2; LAMPB; LGP110