MAP1LC3A Rabbit Polyclonal Antibody

CAT#: TA384696

LC3A Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human stomach, using LC3A Antibody.

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  Immunofluorescent analysis of Hela cells, using LC3A Antibody .

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  Western blot analysis of LC3A expression in Hela cell lysate.

Specifications

Product Data

• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  ICC/IF: 1/50-1/200
  IP: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human MAP1LC3A
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 16kDa
• Gene Name: microtubule associated protein 1 light chain 3 alpha
• Database Link:
  Entrez Gene 84557 Human
  UniProt ID Q9H492
• Background: Swiss-Prot Acc.Q9H492.Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
• Synonyms: LC3; LC3A; MAP1ALC3; MAP1BLC3; OTTHUMP00000030698