MRE11 Rabbit Monoclonal Antibody [Clone ID: R03-6H9]

CAT#: TA384800

Mre11 Rabbit monoclonal Antibody

Specifications

Product Data

• Clone Name: R03-6H9
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human Mre11
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 81 kDa; Observed MW: 81 kDa
• Gene Name: MRE11 homolog A, double strand break repair nuclease
• Database Link:
  Entrez Gene 4361 Human
  UniProt ID P49959
• Background: Swiss-Prot Acc.P49959.Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
• Synonyms: ATLD; HNGS1; MRE11; MRE11B