NANOG Mouse Monoclonal Antibody [Clone ID: 6F5-8F5-4D6]

CAT#: TA384835

Nanog (6F5) Mouse monoclonal Antibody

Specifications

Product Data

• Clone Name: 6F5-8F5-4D6
• Applications: IF, WB
• Recommended Dilution: WB: 1/500-1/2000
  ICC: 1/200-1/1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Ascitic Fluid
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 35kDa
• Gene Name: Nanog homeobox
• Database Link:
  Entrez Gene 79923 Human
  UniProt ID Q9H9S0
• Background: Swiss-Prot Acc.Q9H9S0.NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
• Synonyms: FLJ12581; FLJ40451; hNanog