NELFE Rabbit Monoclonal Antibody [Clone ID: R02-7E5]

CAT#: TA384853

NELFE Rabbit monoclonal Antibody

Specifications

Product Data

• Clone Name: R02-7E5
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human NELFe
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 43 kDa; Observed MW: 43 kDa
• Gene Name: negative elongation factor complex member E
• Database Link:
  Entrez Gene 7936 Human
  UniProt ID P18615
• Background: Swiss-Prot Acc.P18615.Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
• Synonyms: D6S45; NELF-E; RD; RDBP; RDP