MTH1 (NUDT1) Rabbit Monoclonal Antibody [Clone ID: R06-2K9]

CAT#: TA384902

MTH1 Rabbit monoclonal Antibody

Product Images

Western blot detection of MTH1 in K562,Hela cell lysates using MTH1 Rabbit mAb(1:1000 diluted).Predicted band size:23kDa.Observed band size:18kDa.

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  Immunocytochemistry of MTH1(green) in Hela cells using MTH1 Rabbit mAb at dilution 1/50, and DAPI(blue)

Specifications

Product Data

• Clone Name: R06-2K9
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/2000
  ICC/IF: 1/100
  IP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human MTH1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 23 kDa; Observed MW: 18 kDa
• Gene Name: nudix hydrolase 1
• Database Link:
  Entrez Gene 4521 Human
  UniProt ID P36639
• Background: Swiss-Prot Acc.P36639.Antimutagenic (PubMed:8226881, PubMed:7713500, PubMed:10608900). Plays a redundant role in sanitizing oxidized nucleotide pools, such as 8-oxo-dGTP pools (PubMed:28679043). Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress (PubMed:12857738, PubMed:24695224, PubMed:24695225). Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions (PubMed:8226881, PubMed:10373420, PubMed:10608900, PubMed:11756418, PubMed:12857738, PubMed:16607562, PubMed:24695224, PubMed:24695225, PubMed:26999531, PubMed:28035004). Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP (PubMed:10373420, PubMed:11139615). Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA .
• Synonyms: 8-oxo-dGTPase; MTH1; OTTHUMP00000115537; OTTHUMP00000115540