oprk1 Rabbit Polyclonal Antibody

CAT#: TA384911

Phospho-kappa Opioid Receptor (Ser369) Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:1(4°,overnight). High-pressure and temperature Tris-EDTA,pH8. was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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  Western Blot analysis of various cells using Phospho-KOR-1 (S369) Polyclonal Antibody diluted at 1：1000

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  Western Blot analysis of Rat-kidney cells using Phospho-KOR-1 (S369) Polyclonal Antibody diluted at 1：1000

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  Western blot analysis of lysates from NIH/3T3 cells, using KOR-1 (Phospho-Ser369) Antibody. The lane on the right is blocked with the phospho peptide.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  ELISA: 1/10000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from mouse KOR-1 around the phosphorylation site of Ser369. AA range:331-380 (Phosphorylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):42
• Database Link:
  UniProt ID P41145
• Background: Endogenous opioid peptides and opiates, like morphine, transmit their pharmacological effects through membrane bound opioid receptors. Pharmacological studies and molecular cloning have led to the identification of three different types of opioid receptor, mu-type, delta-type and kappa-type, also designated MOR-1, DOR-1 and KOR-1, respectively. MOR-1 is a receptor for beta-endorphin, DOR-1 is a receptor for enkephalins, and KOR-1 is a receptor for dynorphins.The three opioid receptor types are highly homologous and belong to the superfamily of G-protein-coupled receptors. Opioid receptors have been shown to modulate a range of brain functions, including instinctive behavior and emotions. This regulation is thought to involve the inhibition of neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance.