P4HB Rabbit Monoclonal Antibody [Clone ID: R09-1F4]

CAT#: TA384917

P4HB Rabbit monoclonal Antibody

Product Images

Western blot detection of P4HB in K562,C6 cell lysates using P4HB Rabbit mAb(1:1000 diluted).Predicted band size:57kDa.Observed band size:57kDa.

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  Immunohistochemistry of P4HB in paraffin-embedded Human lung cancer tissue using P4HB Rabbit mAb at dilution 1/20

Specifications

Product Data

• Clone Name: R09-1F4
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human P4HB
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 57 kDa; Observed MW: 57 kDa
• Gene Name: prolyl 4-hydroxylase subunit beta
• Database Link:
  Entrez Gene 5034 Human
  UniProt ID P07237
• Background: Swiss-Prot Acc.P07237.This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).
• Synonyms: DSI; ERBA2L; GIT; P4Hbeta; p55; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB