PAH Rabbit Monoclonal Antibody [Clone ID: R02-8B1]

CAT#: TA384922

polyclonal AntibodyPN1 Rabbit monoclonal Antibody

Product Images

Western blot detection of PABPN1 in Jurkat,Hela,COS7,Raji cell lysates using PABPN1 Rabbit mAb(1:1000 diluted).Predicted band size:33KDa.Observed band size:50KDa.

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  Immunohistochemistry of PABPN1 in paraffin-embedded Human breast cancer tissue using PABPN1 Rabbit mAb at dilution 1/20

Specifications

Product Data

• Clone Name: R02-8B1
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000-1/5000
  IHC: 1/20-1/100
  ICC/IF: 1/20-1/50
  IP: 1/20
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human PABPN1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 33 kDa; Observed MW: 50 kDa
• Database Link:
  UniProt ID Q86U42
• Background: Swiss-Prot Acc.Q86U42.Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product . Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length . Increases the affinity of poly(A) polymerase for RNA . Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity . May protect the poly(A) tail from degradation . Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).