PAK1 Rabbit Monoclonal Antibody [Clone ID: R04-2A4]

CAT#: TA384923

Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Rabbit monoclonal Antibody

Product Images

Western blot detection of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) in C6,3T3,Hela cell lysates using PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) Rabbit mAb(1:1000 diluted).Predicted band size:61kDa.Observed band size:61kDa.

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  Immunocytochemistry of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154)(green) in Hela cells using PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of Phospho-PAK1(Ser144)/2(Ser141)/3 (Ser154) in paraffin-embedded Human breast cancer tissue using Phospho-PAK1(Ser144)/2(Ser141)/3 (Ser154) Rabbit mAb at dilution 1/2

Specifications

Product Data

• Clone Name: R04-2A4
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/20-1/100
  ICC/IF: 1/50
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1 (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 61 kDa; Observed MW: 61 kDa
• Database Link:
  UniProt ID O75914
• Background: Swiss-Prot Acc.O75914.Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.