POT1 Rabbit Monoclonal Antibody [Clone ID: R06-9I1]

CAT#: TA385011

POT1 Rabbit monoclonal Antibody

Product Images

Western blot detection of POT1 in K562,C6,3T3 cell lysates using POT1 Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:71kDa.

Specifications

Product Data

• Clone Name: R06-9I1
• Applications: WB
• Recommended Dilution: WB: 1/1000-1/5000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human POT1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 71 kDa; Observed MW: 71 kDa
• Gene Name: protection of telomeres 1
• Database Link:
  Entrez Gene 25913 Human
  UniProt ID Q9NUX5
• Background: Swiss-Prot Acc.Q9NUX5.Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.
• Synonyms: DKFZp586D211; hPot1; OTTHUMP00000211676