Cyclophilin F (PPIF) Rabbit Monoclonal Antibody [Clone ID: R02-6I9]

CAT#: TA385023

Cyclophilin F Rabbit monoclonal Antibody

Product Images

Western blot detection of Cyclophilin F in K562,3T3,Hela cell lysates using Cyclophilin F Rabbit mAb(1:1000 diluted).Predicted band size:22kDa.Observed band size:18kDa.

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  Immunohistochemistry of Cyclophilin F in paraffin-embedded Human tonsil using Cyclophilin F Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R02-6I9
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/500
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Cyclophilin F
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 22 kDa; Observed MW: 18 kDa
• Gene Name: peptidylprolyl isomerase F
• Database Link:
  Entrez Gene 10105 Human
  UniProt ID P30405
• Background: Swiss-Prot Acc.P30405.PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.
• Synonyms: Cyp-D; CYP3; FLJ90798; hCyP3; MGC117207; PPIase; rotamase